Monday, October 4, 2010

Evaluatiuon of different extraction and cell rupture methods for PHA isolation from Rhodospirillum rubrum


Hot Chloroform Extraction Lysozyme Sonication French Press Hypochlorite
PHA content (% dry weight) 13.6 13.6 13.6 13.6
Isolated PHA (% dry weight) 12.5 9.7 7.7 12.1
Recovery % 92 71 58 89
Mw (x 10-6) 1.5 0.92 0.65 0.94
Mn (x 10-6) 1.1 0.36 0.46 0.37
Mw / Mn (Polydispersity) 1.4 2.6 1.4 2.5
Market for Biodegradable Material -
During the early 1990s, annual production of PHAs was just several hundred tonnes. In 1996 Zeneca, the main producer has stopped producing PHBV and sold the assets to Monsanto and Astra. Currently, the market for biodegradable material is largest in Scandi navia and other European countries, where companies are willing to pay up to pounds 4 per kg for PHAs. The estimate given in table below is based on the development of a composting infrastructure, which is now becoming highly developed in many parts of Eu rope, particularly in Germany, Holland and Belgium.
Western European market estimates for biodegradable plastics (tonnes per annum) -
Applications Conventional Resins Biodegradable Resins
Waste Disposal Bags for Composting - 30 000
Disposable Fast Food Utensils 100 000 50 000
Hygiene Films 110 000 20 000
Paper Coatings 420 000 40 000
Agricultural Sector 65 000 30 000
Total 695 000 170 000
Source - BASF

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